A NEW SIMPLE METHOD FOR DETERMINING VIABILITY OF YEAST CELLS VIA DYE EXCLUSION ASSAY

Manoj Kumar,* Rakesh Ranjan, Julian Ekka, Manoranjan Prasad Sinha and Bharti Singh Raipat

ABSTRACT

Aim:
This article describes a novel, easy to use technique for using the dye exclusion experiment to assess the
vitality of yeast cells. Methods: Yeast cells were propagated and suspende d in a solution of trypan blue and
phosphate buffered saline. After that, the suspension was put into the Neubauer chamber for analysis. Results: The
living cells showed no color; that is, they repelled the dye, whereas the dead cells looked blue, since th e cell
membrane of dead cells allowed the entry of the dye. The viable (live) cells present in a suspension can be
quantified following an exclusion test employed in this article. The outcome is based on the fact that intact cell
membranes in living cells prevent the entry of certain dyes, such trypan blue. Conclusion: In this paper, we report
a simple trypan blue dye exclusion method to determine the viability of yeast cells.

Keywords: yeast, dye exclusion assay, trypan blue, viability test.