EFFICACY OF SODIUM HYPOCHLORITE 5.2% ON ENTEROCOCCUS FAECALIS AND IT'S DIAGNOSIS WITH ANTIBIOGRAM ISOLATED FROM INFECTED PULP

Alaa' Turki Monawer Algurairy*

ABSTRACT

Sodium hypochlorite has been used as an endodontic irrigant for more than 70 years, and is now one of the most common solutions for this purpose. Sodium hypochlorite is commonly used for irrigation of root canals in concentrations ranging from 1 to 5.2 %. This study purposed to investigate the antimicrobial efficacy of the Sodium hypochlorite NaOCl 5.2% on isolated E. faecalis from single rooted canals in Duhok city, Kurdistan province, Iraq. For this purpose two hundred extracted single rooted teeth were selected for this study. The root canals were prepared and shaped with sterile reamers (#15-55) and then the canal irrigation with sodium hypochlorite 5.2%,and microbiological samples were collected from each tooth using sterile paper points before and after the treatment with Sodium hypochlorite NaOCl 5.2% and inoculated into(brain heart infusion agar and blood agar). The bacterium was identified using colony morphology, Gram stain, biochemical tests and selective differential media. Further diagnosis confirmation and antibiotic susceptibility were done by BD Phoenix 100. Sixteen (8%) isolates of Enterococcus faecalis were isolated from 200 single rooted canals, after treatment with Sodium hypochlorite NaOCl 5.2% 11 (68.75%)isolates were eradicated, whereas only 5 (31.25%) isolate was resistant to treatment with Sodium hypochlorite NaOCl 5.2%. Before sodium hypochlorite 5.2% treatment, the percentage of E. faecalis isolates susceptible to nitrofurantoin was (93.75%), ampicillin and linezolid (65.25%), vancomycin and teicoplanin (50%). Resistant rate was observed with gentamicin (62.5%), but after sodium hypochlorite 5.2% treatment, the percentage of E. faecalis isolates susceptible to nitrofurantoin was (08%), and (60%) to the rest (gentamicin, ampicillin, teicoplanin, vancomycin and linezolid). This study highlighted that treatment with sodium hypochlorite 5.2% was effective in the elimination of E. faecalis isolated from infected rooted canals. And also BD phonix 100 system proved to be active in the identification and antibiotic susceptibility of E. faecalis.

Keywords: Enterococcus faecalis, dental pulp, Sodium hypochlorite NaOCl 5.2%, BD phonix 100 system.