A REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF LAMIVUDINE BY RP-HPLC METHOD

B. Satya Prasad*, J. N. Suresh Kumar, M. Chandra Varshini, I. V. S. Lasya, B. Deepak, B. Mahesh Naik and K. Ramya

ABSTRACT

A simple, rapid, accurate and precise reverse phase high performance liquid chromatography (RP- HPLC) method was developed for separating and quantifying lamivudine in pharmaceutical dosage forms. Using mobile phase, the isocratic chromatographic separation of lamivudine was performed using Hypersil BDS C18 (150mm, 4.6mm, 5m). This separation was carried out by using mobile phase consisting of mixed phosphate buffer: Acetonitrile at 60;40 v/v and a 20l injection volume with a 1ml/min flow rate and a detecting wavelength of 274nm. The afore mentioned technique was validated with respect to its linearity, robustness, ruggedness, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ). Thus, the developed technique can be used for regular lamivudine quality control.

Keywords: Lamivudine RP-HPLC, Method validation, method development.