SPASMOLYTIC ACTIVITY OF METHANOL LEAF EXTRACT OF LUPINUS ARBOREUS ON GUINEA PIG ILEUM.
*Ohadoma S. C., Osuala F. N. and Nnatuanya I. N.
Aim: To investigate the spasmolytic activity of Lupinus arboreus on guinea pig ileum. Methods: The crude
methanol extract (CME) of the dried leaves was obtained by 72 h cold maceration. The intestinal contents were
removed by flushing with Tyrode’s solution. The tissue was mounted in a 50 ml Organ bath containing Tyrode’s
solution, maintained at 35oC and aerated with air. A load of 0.5 g was applied. Response was recorded
isometrically using Ugo Basile Unirecorder 7050. The CME, and in the presence of atropine (1 X 10-5 g/ml) and in
the presence of atropine (1 X 10-5 g/ml), responses served as control. Result: The CME showed contractile effect
on the guinea pig ileum; while the contractile effect was inhibited by atropine and reduced in the presence of
epinephrine. Conclusion: The contractile effect suggest that Lupinus arboreus may not be effective in the
management of colic pain.
Keywords: Spasmolytic activity, Lupinus arboreus, Chikadoma, Guinea pig ileum, Leaf extract.
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