DEVELOPMENT AND INVITRO CHARACTERIZATION OF CLARITHROMYCIN LOADED CHITOSAN NANOPARTICLES

Nagaraju Shamanthula* and Dr. Alladi Saritha

ABSTRACT

The present study was aimed to develop a nanoparticulate drug delivery system of Clarithromycin using polymer
(Chitosan). The polymer enhances the binding of Clarithromycin nanoparticles in specific or targeted site with
sustained release of drug increasing therapeutic efficacy. These nanoparticles may also reduce the dose frequency
with desired therapeutic response. All batches of nanoparticles (F1-F10) were prepared by nano precipitation
method. The entrapment efficiency of the optimized formulation F7 (drug 50mg, Chitosan 75mg, β –cyclodextin
10 mg) was 99.38 ±0.08 and invitro drug release was 98.46% after 24 hours. It also obey the zero order, follows
diffusion and erosion mechanism of release. Surface morphology of optimized formulation (F7) indicated that
lrbesartan nanoparticles were found to be in average nanometer range(358.4nm) and showed ideal surface
morphology. The stability test performed revealed that the formulation (F7) showed no change in its characters.
The optimized formulation (F7) was also examined for zeta potential determinations. The formulation(F7) showed
maximum deviation of 9.16 mV which demonstrated that the particles are separate and highly repelling property
found to be more useful in decreasing opsonization and favors target specificity. The developed Clarithromycin
nanoparticle formulation increases water solubility, reduces the dose frequency and improves the bioavailability of
drug.

Keywords: Nanoparticulate, Drug Delivery System, Clarithromycin, Chitosan.