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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57



Nada H. Binhashim and Muhammad M. Hammami*


A simple and precise reversed-phase high performance liquid chromatographic (HPLC) assay for the measurement of mefenamic acid level in human plasma, using diclofenac as an internal standard (IS), was developed and validated. Plasma samples containing mefenamic acid were spiked with the IS then extracted with acetonitrile and reconstituted in mobile phase. The compounds of interest were efficiently separated on Atlantis dC18 column at room temperature and detected with photodiode array detector set at 278 nm. The mobile phase consisted of 0.025 M dibasic potassium phosphate (pH = 6.0, adjusted with phosphoric acid) and acetonitrile (65:35, v:v) and was delivered at a flow rate of 1.5 ml/min. The relationship between mefenamic acid concentration in plasma and peak area ratio of mefenamic acid/ IS was linear (R2  0.9987) in the range of 0.05 – 10 μg/ml, the intra- and inter- day coefficient of variations (CV) were ≤ 5.3% and ≤ 7.2%, respectively, and the intra- and inter- day bias was ≤ 6% and ≤ 8%, respectively. Mean extraction recovery of mefenamic acid and the IS from plasma samples was 99% and 92%, respectively. The method was used to assess the stability of mefenamic acid in human plasma under various conditions encountered in the clinical laboratory. Mefenamic acid was stable for 8 weeks at -20°C (≥ 95%), 24 hours at room temperature (≥ 96%) and after 3 freeze-thaw cycles (≥ 96%) in unprocessed plasma samples, and for 48 hours at -20°C (≥ 90%) and 24 hours at room temperature (≥ 92%) in processed samples.

Keywords: Mefenamic acid, Diclofenac, Human plasma, HPLC.

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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia