PHARMACOGNOSTIC SCREENING AND HYPNOTIC ACTIVITY OF LEAVES EXTRACT OF ADENIUM OBESUM
*Vinod Kumar, Sudha Singh and Dr. Shamim Ahmad
ABSTRACT
Insomnia is a problem with the depth or duration of sleep, and difficulties initiating or maintaining sleep are frequent symptoms. The research focuses on the phytochemical and hypnotic screening of leaves extract of A. obesum in experimental animal models. The fresh leaves of A. obesum were collected from Meerut region and authenticated by the botanist. The leaves were washed to make these dust-free; dried in shade. Ethanol (70%) was used as solvent in extraction. 200ml of ethanol (70%) was used for 50gm quantity of powder. Various phytoconstituents were looked for in the plant extracts. Animal House at the Translam Institute of Pharmaceutical Education and Research in Meerut will supply Wistar rats of either sex weighing between 150 and 200g. There are four sets of animals (n=6) i.e., group 1 administered distilled water; group 2 administered Diazepam (4 mg/kg) orally; group 3 administered A. obesum methanolic leaf extract (100mg/kg) orally; group 4 administered A. obesum methanolic leaf extract (200mg/kg) orally once daily for 21 days. Various parameters including Thiopental sod. induced sleeping time, Motor behaviour, Light and Dark Arena and Actophotometer were evaluated for hypnotic activity. Based on dried extract, the percentage yield was calculated as 69.26% when weight of practical yield was compared with theoretical yield. Leaves exhibited alkaloid, flavonoids, glycosides, amino acids as chemical constituents. It also exhibited for tannins in some tests. In results, it was found that A. obesum methanolic leaves extract is very significant in the preclinical treatment of insomnia. Its mode of action might involve inhibiting neurotransmitter release and encouraging catecholamine breakdown. In conclusion, Adenium obesum's methanolic leaf extract is effective in reducing insomnia and resetting the mind to promote sleep. It suggests conducting clinical research to assess the hypnotic effects of A. obesum, which would have been utilized to treat insomnia and other associated issues.
Keywords: Adenium obesum, hypnotic activity, diazepam, thiopental sodium induced sleeping time, locomotor activity.
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