COMPARATIVE STUDY OF ORGANOPHOSPHATE PESTICIDE (METHYL PARATHION) EFFECTS ON ESTERASE ISOZYME IN GILL TISSUE OF HETEROPNEUSTES FOSSILIS AND CHANNA PUNCTATUS
T. Bheem Rao*, P. Subhashini and Kandala Satyanarayana
ABSTRACT
The present study was under taken to assess the toxicological effect of Methyl Parathion (an Organophosphate) on esterase isozyme banding patterns in gill tissue of freshwater cat fish Heteropneustes fossilis (Bloch) and Channa puntatus at different time intervals i.e. 24,48,72 and 96hrs and was compared with control. The esterase isozymes were quantitatively analyzed by using 7.5٪ native polyacrylamide gel electrophoresis (PAGE) stained with α-naphthyl acetate as substrate. Three different esterase bands were detected and named as Est-1; Est-2 and Est-3 with different relative mobilities such as 0.60; 0.40; 0.30 in gill tissue of Heteropneustes fossilis and Three different esterase bands were detected and named as Est-1; Est-2 and Est-3 with different relative mobilities such as 0.60; 0.40; 0.30 in gill tissue of Channa puntatus . Est-1 is deeply stained, Est-2 is moderately stained, Est-3 is faintly stained and in both fishes. Two fishes exhibits Decreasing of Esterase bands intensity is observed.
Keywords: Esterase, isozymes, PAGE, H. fossilis, Channa puntatus Methyl Parathion, Gill Tissue.
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