EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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ISSN 2394-3211

Impact Factor: 7.065

ICV - 79.57

Abstract

INHIBITOR EFFECT OF AQUEOUS EXTRACT OF TRUNK BARK OF SCLEROCARYA BIRREA (ANACARDIACEAE) AND ITS FRACTIONS ON ?-GLUCOSIDASE AND ?-AMYLASE

*Anderson-Bel Justin Kouehiouon Djiké, Ouga Stanislas Zahoui, Goh Bi Lané Adrien, Christelle N’dah Etchien Ekra, Semi Anthelme Néné Bi and Flavien Traoré Félix Houphouet-Boigny University, UFR Biosciences, Laboratory of Biology and Health, Abidjan, Côte D’Ivoire

ABSTRACT

Introduction: Diabetes mellitus is one of the leading causes of death worldwide. One of the therapeutic approaches for this metabolic pathology consists of inhibiting digestive enzymes. The objective of this work is to evaluate the inhibitory properties of α-amylase and α-glucosidase of the aqueous extract of trunk bark of Sclerocarya birrea and its organic fractions. Methods: In vitro, the aqueous extract, its ethanolic fraction, its cyclohexanic fraction, and its ethyl acetate fraction at concentrations of 200; 400; 600; 800; 1000; 1500 and 2000 μg/mL are preincubated with α-amylase (2%) or α-glucosidase (2%) in buffer solution for 5 min. After pre-incubation, substrate is added to each test tube and then incubated for 30 min. The enzymatic reaction is stopped by the addition of 3,5 dinitrosalicylic acid (DNS). The Test was repeated three (3) times. Results: The aqueous extract of trunk bark of Sclerocarya birrea and its different fractions at concentrations of 200; 400; 600; 800; 1000; 1500 and 2000 μg/mL inhibit digestive enzymes in a concentration-dependent manner with 50% inhibitory concentrations (IC50) of 885; 1130; 461; and 1328 μg/mL respectively for the aqueous extract, its ethanolic fraction, its cyclohexanic fraction, and its ethyl acetate fraction. The F4 fraction and the total extract presented the best inhibitory activities. Conclusion: The aqueous extract and its cyclohexanic fraction by inhibiting digestive enzymes can reduce the postprandial increase in blood glucose and therefore be an effective approach to manage blood glucose levels in diabetes mellitus.

Keywords: Diabetes, ?-glucosidase, ?-amylase Sclerocarya birrea.

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