ALLEVIATING CYPERMETHRIN-INDUCED HYPOTHYROIDISM AND HEMATOLOGICAL CHANGES IN FEMALE SWISS ALBINO MICE USING BACOPA MONNIERI

Surbhi Chourasiya and Dr. Versha Sharma*

ABSTRACT

Hypothyroidism is a common outcome resulting from the interaction of various drugs, environmental pollutants (insecticides), and industrial chemicals with the endocrine glands. The thyroid gland is an important endocrine gland in the human body. Thyroid hormones play a crucial role in proper development, metabolic balance, differentiation, and physiological function of nearly all tissues. The thyroid gland produces hormones such as triiodothyronine (T3) and tetraiodothyronine or thyroxine (T4). Under physiological condition, thyroid hormone influences hematological parameters. Cypermethrin is a type II synthetic pyrethroid insecticide used worldwide to reduce crop loss and pest population. Although Bacopa monnieri has been used for various diseases, it is best recognized as an antioxidant and also for brain-boosting properties. This study was done to evaluate the effect of cypermethrin-induced Hypothyroidism on different hematological parameters and the ameliorative effect of Bacopa monnieri. Female Swiss albino mice were used in the investigation and were divided randomly into four groups viz. Group I control, Group II cypermethrin (15mg/kg body weight) treated, Group III cypermethrin and Bacopa monnieri treated (15mg/kg body weight, and 200mg/kg body weight simultaneously), and Group IV Bacopa monnieri administered (200mg/kg body weight) via oral gavage for 28 days. Results indicate that the Cypermethrin induced-hypothyroidism group II showed significant alterations in hematological parameters suggesting that significantly increased WBC and Platelets count, likewise a significantly decreased RBC, and Hb% lead to anemia, are all associated with hypothyroidism, whereas thyroid hormonal parameters also showed significantly decreased serum T3 and T4 (***p<0.001) and increased TSH (***p<0.001) levels are shown in induced- hypothyroidism group. The Bacopa monnieri showed therapeutic efficacy in the BM co-administered group when compared to control and treated groups. The investigation showed the elucidating potential of bacopa monnieri on hematological alterations in Female Swiss albino mice associated with CYP-induced hypothyroidism. The study revealed that thyroid diseases impacted females more frequently than males. Emphasizing a strong link between hematological parameters and thyroid dysfunction in mice. The medicinal application of this plant and its numerous possibilities for further investigation remain in relatively new areas of study. Analyzing the chemical composition of this plant will enable further exploration of its remediation and hematological effects in mice.

Keywords: Hemoglobin, Hypothyroidism, Hematological Parameter, Cypermethrin, Bacopa monnieri.