SIMILARITY BASED DESIGN, SYNTHESIS AND CHARACTERIZATION OF ISONIAZID ANALOGUES AND THEIR THERAPEUTIC POTENTIAL

Anjali Dinesh*, Ashique Palakkathondi*, Nurul Ameen, Manju C. S. and Muhammed Riyaz Pulikkal, Dr. Sreena K.

ABSTRACT

The alarming rise of Mycobacterium tuberculosis (Mtb) strains that are multi-drug resistant has prompted the scientific community to scramble for fresh, effective anti-tubercular treatments. Despite the different medications that are now being studied, isoniazid remains the most important and efficient part of all multi-therapeutic regimens that the WHO recommends. The computational design, synthesis, and in vitro anti-tubercular activity of a number of potent isoniazid derivatives against H37Rv are all covered in this study. In this study, docking tests were done to determine the biological activity of three isoniazid derivatives that were created. A simple and efficient method was developed for the synthesis of designed compounds and the structures of the compounds were characterized by means of their FT-IR, 1H NMR, 13C NMR and mass spectrometry. The anti-tubercular activity of the compound with the relatively high binding affinity for the chosen target (2NSD) was evaluated. Microplate Alamar Blue Assay (MABA) was used to measure the anti-tubercular activity. According to the experimental findings, compound IBF1 demonstrated positive anti-tubercular activity with a MIC of 3.12 g/ml.

Keywords: Isoniazid, Computational design, In-vitro anti-tubercular activity, Microplate Alamar Blue Assay.