DETECTION OF HELICOBACTER PYLORI FROM THE GASTRIC BIOPSY SAMPLES OF THE SUSPECTED PEPTIC ULCER DISEASE PATIENTS BY CULTURE AND NESTED PCR

Swarna Paul*, Md. Ehsanul Hoque, A. M. A. M. Zonaed Siddiki, Arup Kanti Dewanjee, Ershad Uddin Ahmed, Abul Kalam, Saurav Das and Showmik Paul

ABSTRACT

Background: One of the most prevalent persistent human illnesses is caused by Helicobacter pylori (H. pylori). Peptic ulcer disease, gastric cancer, and gastric mucosa-associated lymphoid tissue lymphoma are all firmly linked to the infection, which plays a crucial role in the progression of various gastro-duodenal disorders. Aim: Our purpose was to examine the diagnostic compatibility of culture and Nested PCR and to create a useful comparison. Method: Patients undergoing upper gastrointestinal endoscopy at the Gastroenterology Department of the Chittagong Medical College and Hospital, Chattogram were included if they met the inclusion and exclusion criteria. The research was conducted from January 2021 to December 2021 by the Department of Microbiology at Chittagong Medical College in Chattogram. A total number of 110 individuals with dyspeptic symptoms, who underwent upper GI endoscopy, were included. Results: Two stomach antrum samples from each patient were taken for culture and Nested PCR analysis independently. Of the 110 patients tested for PUD, 74 (67.27%) were positive for cultures, whereas 32 (32.73%) tested negative. 82 (74.55%) tested positive by Nested Polymerase Chain Reaction, and 28 (25.45%) did not. Conclusion: Nested PCR was shown to have a greater sensitivity (97.30%) and accuracy (89.09%) than traditional culture method for detecting H. pylori infection, as shown in the research. In addition, PCR is sensitive, fast, and yields results rapidly.

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