ISOLATION, EXTRACTION, PURIFICATION AND APPLICATIONS OF BIOSURFACTANT PRODUCING BACTERIA
H. Sahana, Harshini R., Lakshmi Badiger, Dr. Siddalingeshwara K. G.* and Prakruthi
ABSTRACT
Biosurfactants are surface active compounds produced by a wide range of microorganisms. They have different chemical structures like – lipopeptides, glycolipids, neutral lipids and fatty acids. The objective of this project was to perform screening of biosurfactant producing microorganisms from different samples like soil and water from different places were used, Soil samples from garage, garden, forest and water samples from sewage treatment plant were used in the study. The isolates were cultured in nutrient broth with tween 80 and their extracts were evaluated according to the drop collapse, oil dispersion, emulsification assay. This present study deals with the isolation, screening, production and purification of bio surfactant produced from bacteria and fungi and its application in biofilm assay, antimicrobial property and oil degradation in water.
Keywords: Bio surfactant, Oil displacement, Emulsification, drop collapse, antimicrobial properties, oil degradation, anti-biofilm activity.
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