EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

IMMUNOPHENOTYPING IN THE DIAGNOSIS AND CLASSIFICATION OF ACUTE LEUKEMIA: NATIONAL ONCOLOGY CENTER, ADEN

Mayson Awadh Akrabi and Gamal Abdul Hamid*

ABSTRACT

Background: Acute leukemia comprises a heterogenous group of malignancies with variable clinical, morphologic, immunophenotypic and, molecular features. Flow cytometry is a crucial tool in the diagnosed and subtype hematological malignancy, especially acute leukemia, determining prognosis and monitoring response to therapy. By detecting various antigens presenting in various parts of cell, it is possible to know cell lineage and immaturity of the cell or group of cells. Objective: To evaluate immunophenotypic patterns of acute leukemia patient by multiparameter flowcytometry that help in the diagnosis and proper classification of acute leukemias. Materials and Methods: A descriptive study of acute leukemia cases was conducted at National Oncology Center Aden in Al -Sadaka Teaching Hospital over one year (January 2015 to June 2016). A total of 55 cases of acute leukemia diagnosed by multi parameter flow cytometry performed on peripheral blood and/ or fresh bone marrow aspirates. Results: 55 cases of acute leukemias were retrieved; 29(52.7%) of them were acute lymphoblastic leukemia (ALL), were B cell type (n=27) more than T cell type (n=2), and the remainder 26(47.3%), were proved by flowcytometry to be acute myeloblastic leukemia subtypes and one was acute myeloblastic leukemia (AML) with mixed phenotype (biphenotypic). Progenitors markers (CD34, HLA-DR, CD117 and TdT) were expressed more in acute myeloblastic leukemia more than in acute lymphoblastic leukemia blast cells, except TdT which was expressed by 13.8% of acute lymphoblastic leukemia (ALL) patients but not in acute myeloblastic leukemia patients. The B-lineage markers that expressed with higher percentage among ALL patients included CD19, CD10 and CD79a. Followed by CD20 and CD22. Only 2 patients with ALL expressed CD7 and cytoplasmic CD3 at the same times. Among the T-lineage markers, CD7 was aberrantly expressed in 26.9% and CD19 among B-lineage markers expressed 30.8% of AML patients. Conclusion: In our study the markers that expressed with higher percentage among ALL patients included CD19, CD10 and CD79a. The myeloid markers that were expressed markedly in AML patients included CD13, CD33 and cytoplasmic MPO.

Keywords: Acute leukemia; Immunophenotyping flowcytometry.


[Full Text Article] [Download Certificate]

Citation

  All Since 2019
 Citation  3086  2560
 h-index  18  17
 i10-index  64  44

INDEXING

NEWS & UPDATION

  • EJPMR New Impact Factor

    EJPMR Impact Factor has been Increased to 7.065 for Year 2024.

  • EJPMR: FEBRUARY ISSUE PUBLISHED

    FEBRUARY 2024 issue has been successfully launched on 1 FEBRUARY 2024.

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 February 2024 Issue has been Published, Kindly check it on https://www.ejpmr.com/issue

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia