HPLC FINGER PRINTS OF PHENOLIC COMPOUNDS PRESENT IN ACTINIOPTERIS RADIATA (SWARTZ) LINK.

R. Manonmani* and S. Catharin Sara

ABSTRACT

The present investigation was carried out to analyze the phenolic or flavonoid components from the whole plant of Actiniopteris radiata (Pteridaceae) using HPLC technique. The results clearly showed the presence of five phenolic compounds such as Gallic acid (GA), Caffeic acid(CA), Rutin(RU), Quercetin(QU) and Ferulic acid(FA). The five peaks were observed at 280nm and these five fractions were collected at different retention time (Rt), peak area, height and concentration.The HPLC chromatogram of the five peaks of the flavonoid compounds detected and the results based on the retention time such as Gallic acid (Rt-5.967), Caffeic acid (Rt-9.600), Rutin (Rt-10.525), Quercetin (Rt- 12.292) and Ferulic acid (Rt-23.917) in A. radiata were found to be in the concentration of 0.5 μg/gm, 0.90 μg/gm, 2.0 μg/gm, 2.0 μg/gm, and 0.1 μg/gm respectively. The obtained values were compared with the chromatographic separations of standards. The Individual flavonoid content for all the flavonoids were calculated from the corresponding calibration curve, plotted and presented as validation data. The biological activities of phenolic or flavonoid compounds were found to be effective antimicrobial substances against a wide range of microorganisms. The HPLC fingerprints of the compounds could be serve the purpose of established benchmarks for future plant research.

Keywords: Actiniopteris radiata, HPLC analysis, phenolic components, flavonoids, standards.