EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57

Abstract

HPLC FINGER PRINTS OF PHENOLIC COMPOUNDS PRESENT IN ACTINIOPTERIS RADIATA (SWARTZ) LINK.

R. Manonmani* and S. Catharin Sara

ABSTRACT

The present investigation was carried out to analyze the phenolic or flavonoid components from the whole plant of Actiniopteris radiata (Pteridaceae) using HPLC technique. The results clearly showed the presence of five phenolic compounds such as Gallic acid (GA), Caffeic acid(CA), Rutin(RU), Quercetin(QU) and Ferulic acid(FA). The five peaks were observed at 280nm and these five fractions were collected at different retention time (Rt), peak area, height and concentration.The HPLC chromatogram of the five peaks of the flavonoid compounds detected and the results based on the retention time such as Gallic acid (Rt-5.967), Caffeic acid (Rt-9.600), Rutin (Rt-10.525), Quercetin (Rt- 12.292) and Ferulic acid (Rt-23.917) in A. radiata were found to be in the concentration of 0.5 μg/gm, 0.90 μg/gm, 2.0 μg/gm, 2.0 μg/gm, and 0.1 μg/gm respectively. The obtained values were compared with the chromatographic separations of standards. The Individual flavonoid content for all the flavonoids were calculated from the corresponding calibration curve, plotted and presented as validation data. The biological activities of phenolic or flavonoid compounds were found to be effective antimicrobial substances against a wide range of microorganisms. The HPLC fingerprints of the compounds could be serve the purpose of established benchmarks for future plant research.

Keywords: Actiniopteris radiata, HPLC analysis, phenolic components, flavonoids, standards.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing