STUDY ON PHYTOCHEMICAL COMPOUNDS AND METALLIC NANO PARTICLES PRESENT IN LEAVES OF HIBISCUS ROSA-SINENSIS, PSIDIUM GUAJAVA AND CALOTROPIS GIGANTEA

Madhuri M.*, Nikita Roy, Pallavi G., Dr. Pramod T., Dr. Siddalingeshwara K. G. and Prakruthi

ABSTRACT

Phytochemistry deals with the study of phytochemicals which are present in natural vegetation like plants and it is used in medicine and therapeutics. In this reasearch conducted, three different medicinal plant samples were taken i.e., Hibiscus rosa-sinensis, Psidium guajava and Calotropis gigantea. These three plants samples extracts were obtained by using different solvents in a Soxhlet apparatus. Nutritional quantification and Phytochemical analysis was performed to identify the presence of carbohydrates, reducing sugars, proteins and phytoconstituent like polyphenols, alkaloids, flavonoids, tannins etc. Determination of Anti-inflammatory was carried out by protein denaturation method where percentage of more hemolysis was seen on Hibiscus rosa-sinensis by 91.91%. Anti-inflammatory activity detection done by Human Red Blood Cell(HRBC) method where percentage of protection was calculated and found out that Psidium guajava have highest anti-inflammatory activity by 81.6%. Determination of Antidiabetic was done by DNSA method where Psidium guajava was found to have highest percentage which is 19.8%. From Leaf samples metallic nanoparticles like copper, silver and iron were synthesized and was purified. Application of this nanoparticles towards antimicrobial activity was done against organisms like E. coli, Pseudomonas, Bacillus and Staphylococcus aureus, Fusarium and Aspergillus was used. Hibiscus rosa-sinensis shows highest antibacterial activity and antifungal activity compared to Psidium guajava and Calotropis gigantea. These studies suggests that Hibiscus rosa-sinensis, Psidium guajava and Calotropis gigantea have the potential to be used in future as medicine for various diseases.

Keywords: Phytochemical properties, plant extract, anti-microbial properties, nanoparticles, Hibiscusrosa-sinensis, Psidium guajavaand Calotropis gigantean.