EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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ISSN 2394-3211

Impact Factor: 7.065

ICV - 79.57

Abstract

ASSESSING THE MOLECULAR GENETIC VARIATION OF SOME LOCAL ISOLATES OF THE FUNGUS BEAUVERIA BASSIANA (BALS.) USING RAPD PCR

Muntadher Alaa Falih, Hayder Shammer Abbas, Noor Jassim M., *Sajid Hamid Eesee and Mohammed Jasim

ABSTRACT

The results of the genetic analysis using 15 primers for RAPD technique showed a difference in the number of bands. The number of polymorphic bands was 127 for all primers, and many of them showed unique bands for some isolates, which are a fingerprint to distinguish the isolates. The isolate 100Bb was the highest number of bands these primers have given 34 bands with different molecular weights. To find the percentage of genetic affinity between fungal isolates, the data were introduced in SPSS program. The results showed a difference in similarity percentage between isolates. The highest was among the isolates 17Bb and 47Bb (0.437) they were the closest genetically and the lowest between 100Bb and 17Bb were 0.039, the results of the dendrogram analysis showed isolation of the isolates into two main groups, the first group included the isolates 17Bb and 47Bb, and in the second group the isolate 100Bb.

Keywords: Beauveria bassiana, RAPD PCR, Molecular Variation.

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