DEVELOPMENT AND VALIDATION OF RP- UPLC METHOD FOR SIMULTANEOUS DETERMINATION OF LAMIVUDINE AND DOLUTEGRAVIR IN COMBINED DOSAGE FORMS
*P. Bala Krishnaiah, Shivaratri Srikanth and Bairam Ravindar and Manjunath S. Y.
ABSTRACT
A simple, accurate, precise method was developed for the simultaneous estimation of the Lamivudine and Dolutegravir in pharmaceutical dosage form by RP-UPLC. Chromatogram was run through HSS C18 (2.8 x 50 mm column at a flow rate of 0.3 mL/min. Buffer used in this method was Potassium dihydrogen phosphate. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Lamivudine and Dolutegravir were found to be 1.408 min and 1.739 min. The percentage RSD of the Lamivudine and Dolutegravir were and found to be 0.8 and 0.8 respectively. The percentage recovery was obtained as 100.39% and 100.37% for Lamivudine and Dolutegravir respectively. LOD, LOQ values obtained from regression equations of Lamivudine and Dolutegravir were 0.41, 1.25 and 0.09, 0.26 respectively. Retention times were decreased and the run time was decreased, so the developed method was simple, economical and effective for the routine quality control test in industries.
Keywords: Lamivudine, Dolutegravir, RP-UPLC.
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