REVIEW ON COMPARATIVE SCREENING OF VARIOUS ANALYTICAL METHODS FOR CEFIXIME TRIHYDTRATE IN PHARMACEUTICAL DOSAGE FORMS

R. Revathi*, V. Ganesan, E. Mohammed Thowfic Raja, M. Premkumar, A. Chelladurai, K. Sudharsan and R. Jothilakshmi

ABSTRACT

Cefixime Trihydrate (CT) is a broad-spectrum, third-generation cephalosporin antibiotic derived semi synthetically from the marine fungus Cephalosporium acremonium with antibacterial activity. As does penicillin, the beta-lactam antibiotic CT inhibits bacterial cell wall synthesis by disrupting peptidoglycan synthesis, resulting in a reduction in bacterial cell wall stability and bacterial cell lysis. Stable in the presence of a variety of beta-lactamases, this agent is more active against gram-negative bacteria and less active against gram-positive bacteria compared to second-generation cephalosporins. It is used in lower respiratory tract infection. It is helpful in acute urinary tract infection, bilary tract infection, sinusitis, acute otitis media, peptic ulcer and many more. Thus, numerous number of research works have been conducted and published to estimate CT because of their biological and pharmacological activity. This review article represents the collection and discussion of various analytical methods available in the literature for the determination of CT in bulk, pharmaceutical dosage forms and biological samples comprising of HPLC, HPTLC, UV-Visible spectroscopic method and hyphenated technique such as LC-MS. This brief review is intended to steer the various analytical methods and recent developments implemented for the analysis of CT content in various dosage forms and in combination with antibiotics, which are indeed being explored for the future analytical study of CT.

Keywords: Cefixime Trihydrate, Comparative Screening, Hyphenated Technique, ICH guidelines.