ASSOCIATION BETWEEN LNCRNA-MALAT1 IN SERUM AND TYPE 2 DIABETES AND IT’S RISK FACTORS: A COMMUNITY-BASED CASE-CONTROL STUDY

Zhiheng Zhou*, MD, PhD; Wenjie Shi*, MD; Xuejiao Zhang, MD, PhD and Jiaji Wang MD.

ABSTRACT

Background Previous studies have shown that long non coding RNA plays an important role in the occurrence and development of diabetes, and experimental studies also shown that LncRNA-MALAT1 was related to type 2 diabetes, but in the population, the correlation between serum LncRNA-MALAT1 expression level and type 2 diabetes has not been clarified. Objective To explore the relationship between serum LncRNA-MALAT1 expression level and the prevalence of type 2 diabetes, and to provide new ideas for exploring risk factors and new biomarkers of type 2 diabetes in the future. Methods 320 patients with type 2 diabetes newly diagnosed were selected as the case group, and 320 healthy people without endocrine system diseases were selected as the control group according to the 1:1 matching principle. The clinical data of these people were collected, and their peripheral blood was collected to detect the diagnostic indicators of diabetes, clinical biochemical indicators, and the expression of LncRNA-MALAT1 in serum by real-time fluorescent quantitative PCR, To analyze the association between human serum LncRNA-MALAT1 and type 2 diabetes, and the relationship between LncRNA-MALAT1 and related risk factors. Results The expression level of serum LncRNA-MALAT1 in the case group (3.24 ± 0.53) was significantly higher than that in the control group (1.124 ± 0.213). In patients with type 2 diabetes, the expression level of serum LncRNA-MALAT1 was positively correlated with fasting blood glucoser (r=0.612) and glycosylated hemoglobin (r=0.523), BMI (r=0.604), TC (r=0.517) and LDL (r=0.678)(P<0.05), but not with other indicators (P>0.05). Multiple logistic regression analysis showed that smoking (OR=2.144), hypertension history (OR=1.981), TC (OR=1.358) and LncRNA-MALAT1 (OR=2.632) were risk factors for type 2 diabetes, while regular exercise (OR=0.562) was a protective factor for diabetes. Conclusion The serum LncRNA-MALAT1 is highly expressed in patients with type 2 diabetes, and has correlation with diabetes related diagnostic indicators and lipid indicators. LncRNA-MALAT1 may be a new biomarker of type 2 diabetes.

Keywords: Type 2 diabetes, long non coding RNA-MALAT1, risk factors, case-control study.