COMPARATIVE STUDY OF SOME IMMUNE INDICATORS IN THE SERUM OF PATIENTS WITH TYPE 1 DIABETES

Dhuha Adnan Neamah*, Zainab A. Fadhil, Fyaa Raad Yaha and Adnan Neamah Abdulridha

ABSTRACT

Type 1 diabetes (T1DM) is achronic autoimmune disease ،characterized by the destruction of pancreatic β-cells, that produce insulin in specific areas of the pancreas, known as islets of Langerhans, leading to insulin deficiency. The study aimed to comparison of levels of IL-6, CRP and Immunoglobulin (IgM, IgG, IgA) in the serum of patients and healthy controls, And study the influence of age and gender on the prevalence of T1DM. the present study was conducted in Baquba Teaching Hospital / Diyala province for the time period from January and the last day of February 2023. A blood sample was taken from 50 volunteers(30 T1DM patients and 20 healthy people) aged between (1-75) years. Serum concentrat of IL-6 indicators within samples were investigated by Sandwich ELISA, the latex agglutination test was used to examine CRP, And measuring the concentration of immunoglobulin (IgA, IgM, IgG) in the serum of patients and healthy people. Results of present study showed there is (1-20) years age group scored highest percentage 43.3% of patients 0(p<0.05). the difference between males than females were no significant (p>0.o05). The results also showed an increase in IL-6 levels in patientsin T1DM patients (71.36±25.09) than healthy (17.11±8.84) with significant different (p<0.05). There was also a significant difference (p<0.05) in the CRP positivity and negativity, between T1DM patients compared to healthy people. The results also showed raised levels of IgG, IgM, IgA in T1DM patients (2116.22±871.70, 2035.27±901.18, 1894.13 ±622.33) than healthy (1916.35±791.96, 242.50±103.01, 432.14±189.82) with significant different (p<0.05). The study found that there are significant differences in the age factor, while there are no clear differences in the gender factor, and that sick samples had higher amounts of IL-6, CRP and immunoglobulin (IgM, IgG,IgA) than healthy control samples.

Keywords: Type 1 diabetes, interleukin-6, C-reactive protein, Immunoglobulin.