EVIDENCE OF THE TOXICITY EFFECT OF TETRACLINIS ARTICULATA ON THE DISEASE VECTOR CULEX PIPIENS FROM MOHAMMEDIA
Souhail Aboulfadl*, Chafika Faraj, Brahim Aouinty and Fouad Mellouki
ABSTRACT
Objective: To determine the larvicidal activities of Tetraclinis articulata (Family Cupressaceae) extracts against Culex pipiens. Methods: Twenty late III instar larvae were exposed to various concentrations (1-3.5 %) of aqueous extract, (1-6 %) of floral water, (50-200 ppm) of essential oil, and were assayed in the laboratory by using the protocol of WHO 2005; the 24 h LC50 values of the Tetraclinis articulata wood extracts was determined following Probit analysis. The susceptibility status of Culex pipiens was determined against four insecticides: temephos, chlorpyriphos, DDT and deltamethrin under the laboratory conditions. Results: Results showed varying degree of larvicidal activity of the three extracts of Tetraclinis articulata against Culex pipiens mosquitoes with LC50 values ranging between 1,3(%) to 295(ppm). The extracts had strong toxicity activity against Cx. pipiens as it provided 100% mortality for 48h of aqueous extract (3.5%), followed by floral water (72 h) at 5% and essential oil (24h) at 200ppm providing 50% of mortality. The susceptibility status against chemical insecticides displayed high resistance to temephos and to chlorpyriphos and a resistance to DDT and deltamethrin. Conclusions: From the results, it can be concluded the extracts of Tetraclinis articulata was an excellent potential for controlling Culex pipiens mosquitoes.
Keywords: The susceptibility status of Culex pipiens was determined against four insecticides: temephos, chlorpyriphos, DDT and deltamethrin under the laboratory conditions.
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