IN VITRO STUDY OF BACOPA MONNIERI AND TAGETES ERECTA EXTRACT-LOADED DOUBLE EMULSION FOR INHIBITORY ALZHEIMER’S DISEASE AND ANTI-CATARACTOGENIC

Supachai Chumchuen*, Somboon Tiranupan, Julalak Kantanalit and Yoskrai Sridhanasakulchai

ABSTRACT

Bacopa monnieri and Tagetes erecta extracts had properties to eliminate dementia and nourish eyes. Bacoside A and rutin are the major chemicals in both extracts, respectively. The study extracted bacoside A and rutin in a specific solvent. The preliminary control extraction by TLC method compares with the standard, study stability of extract-loaded double emulsion, and in vitro inhibition of acetylcholinesterase. The results showed 53.87 % and 46.98% entrapment efficacy of Bacopa monnieri and Tagetes erecta, respectively. This formulation contained an extract of 0.5-2 mg/mL that could be inhibitory acetylcholinesterase significant when compared with blank p > 0.01. However, 0.5 mg/mL of both extracts could inhibit acetylcholinesterase, which is the main reason for Alzheimer’s disease. In the case of HLEB-3 cells, group No. 2 had higher efficacy cell available more than group No.1. Moreover, these formulations were stable at 45 °C for 3 months.

Keywords: Alzheimer’s disease, Bacopa monnieri, Tagetes erecta.