DEVELOPMENT AND VALIDATION OF NEW UV-SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF CIPROFLOXACIN HYDROCHLORIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM
Suresha D.N.*, Rashmi T. and T. Balasubramanian
ABSTRACT
A Novel, specific, accurate, and precise Zero order derivative spectroscopy method was developed and validated for the estimation of Ciprofloxacin HCl in Bulk and pharmaceutical dosage forms. The stock solution was prepared by weighing 100 mg of standard Ciprofloxacin HCl in 100 ml volumetric flask with distilled water. The stock solution was made to produce 1000 μg/ml with distilled water. Further dilutions were prepared as per procedure. The drug solution showed the maximum absorbance at 272 nm. The linearity was found in the concentration range of 2-12 μg/ml. The correlation coefficient was found to be 0.999. The regression equation was found to be Y=0.083x+0.002. The method was validated for linearity, accuracy, precision, LOD, LOQ and ruggedness. The LOD and LOQ for estimation of Ciprofloxacin HCl was found to be 0.049μg/ml and 0.498μg/ml respectively. Recovery of Ciprofloxacin HCl was found to be in the range of 99.22-99.83 %. Proposed method was successfully applied for the quantitative determination of Ciprofloxacin Hydrochloride in Bulk and pharmaceutical dosage forms.
Keywords: Ciprofloxacin HCl, Zero order UV- Spectroscopy, Distilled water, Accuracy.
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