AN EFFICIENT IN VITRO REGENERATION STUDIES OF COMMERCIALLY IMPORTANT GROUND NUT (ARACHIS HYPOGAEA L.) CULTIVARS ICGV15311, ICGV15287, ICGV13074

Swapna Mittapalli, Sumankalyan Sadhu, Jyothi Potta, Arpitha Madadi, Nishath Anjum and Rajender Vadluri*

ABSTRACT

Highly efficient In vitro regeneration protocol was established of ground nut varieties ICGV 15311,ICGV 15287, ICGV13074 seeds from explants ,embryo axis, Half cut embryo axis, leaf and cotyledonary node, various concentrations and combinations of different plant growth regulators (PGRS) were employed to introduce multiple shooting and rooting to obtain a complete plantlets of ground nut on Murashige and skoog (MS) medium supplemented with different concentrations of auxins (IAA,NAA,IBA and2,4 –D) and cytokinins (KIN and BAP). High concentrations of cytokinins and low concentrations of auxins were used for shoot regenerated. The shoot multiplication was influenced by genotype explants type and growth regulators .The explants cultured on KN and 2,4-D Showed on swelling and enlargement without further development. The combinations of BAP and NAA produced more shoots was obtained from embryo axis on a medium containing BAP (2.0mg/L) and NAA (0.5mg/L). Rooting of regenerated shoots was achieved on a medium augmented with IBA (2.0mg/L).Rooted plantlets were successfully transferred to soil and grown in a green house to maturity. ICGV 15311 were responsive with this regeneration system.

Keywords: Embryo axis, Ground nut, Cotyledonary node, Leaf, Shoot regeneration, Root regeneration.