EXPLORING THE ANTI-ULCER POTENTIAL OF ETHANOLIC EXTRACT FROM INDIGOFERA ASPALATHOIDES: COMPARATIVE EVALUATION AND SPECTROSCOPIC INSIGHTS

Krishnamalar G.*, Venkataraman S., Tamizmaran V., Vaithilingam B., Abisesh M., Sakthivel R. and Vinoth Bernando V.

ABSTRACT

Various areas of the body can be affected by ulcers, characterized by open sores or lesions of the skin or mucous membranes. A number of factors can lead to ulcers, such as infections, poor blood circulation, and autoimmune disorders. There are various medicinal systems that use Indigofera aspalathoides (Fabaceae), a plant that grows in diverse geographical regions and is used for treating a wide range of ailments. It is possible to shed light on Indigofera aspalathoides' anti-ulcer potential through extracts like ethanolic extracts. Compounds such as flavonoids, tannins, and other phytochemicals found in this family have displayed various health-promoting effects, including anti-inflammatory and wound-healing properties. The Rf value for the ethanolic extract of Indigofera aspalathoides (Specifically targeting flavonoids) was determined to be 1.11. An FT/IR-4600 type A spectrophotometer with a scanning range of 7800 to 350 cm-1, a resolution of 4 cm-1, and a scanning rate of 10 frames/sec was used to acquire the IR spectra of ethanolic extract of Indigofera aspalathoides. Interestingly, the spectral patterns observed in the stretching vibrations of Omeprazole closely resemble those found in our ethanolic extract of Indigofera aspalathoides. In this study, our objective is to investigate the anti-ulcer activity of Indigofera aspalathoides using the ethanolic extraction method. To induce gastric lesions, an oral dose of 200 mg/kg of acetyl salicylic acid was administered to the rats. Notably, pre-administration of EEIA orally, at doses of both 100 and 200 mg/kg, exhibited significant prevention of gastric ulcer formation. However, the efficacy of EEIA in this regard appeared to be comparatively lower when contrasted with the performance of the standard drug, omeprazole.

Keywords: Indigofera aspalathoides, Anti-ulcer, Aspirin, Pyloric ligation, H.pylori.