ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CEFOXITIN SODIUM FOR INJECTION BY UV VISIBLE SPECTROPHOTOMETRY
R. Suneetha*, D. Varun, C Shirisha, Syed Shafi Uddin, K. Sharath Chandra, Katmode Nikhitha, P. Raju and
S. K. Godasu
ABSTRACT
A rapid, cheap, reliable and simple spectrophotometric method for the quantitive determination of Cefoxitin in powder for injection dosage forms was developed. The proposed method obeys Beer’s law limit in the range of 10 – 50 μg/mL with an apparent molar absorptivity of 8.451 x 103 L mol-1 cm-1. Linear regression of absorbance on concentration gave the equation Y= 0.026X – 0.167, with a correlation co-efficient of R2 = 0.999. The detection wavelength was found at 231 nm. LOD and LOQ were found to be 1.39μg/mL and 4.21μg/mL for Cefoxitin sodium respectively. The accuracy of the method was proved by performing recovery studies which were carried out by analyzing the formulation with three different levels like 10%, 20% and 30%. A value closer to 100% indicates that the proposed method is accurate for the analysis. Thus, it was concluded that the above method was simple, precise and easy to perform and require short time to analyze the drug in the commercial formulations.
Keywords: Cefoxitin Sodium, UV, Method development, Validation.
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