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An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



Rajaa F. Hussein, Nada H. Binhashim, Syed N. Alvi and Muhammad M. Hammami*


A simple and precise reversed-phase high performance liquid chromatographic (HPLC) assay for the measurement of omeprazole level in human plasma, using lansoprazole as an internal standard (IS), was developed and validated. Plasma samples containing omeprazole were spiked with the IS then extracted with tert. butyl methyl ether and reconstituted in mobile phase. The compounds of interest were efficiently separated on Atlantis dC18 column at room temperature and detected with photodiode array detector set at 302 nm. The mobile phase consisted of 0.05 M dibasic sodium phosphate (pH = 7.0, adjusted with phosphoric acid) and acetonitrile (60:40, v:v) and was delivered at a flow rate of 1.0 ml/min. The relationship between omeprazole concentration in plasma and peak height ratio of omeprazole/ IS was linear (R2  0.9992) in the range of 0.01 – 1.20 μg/ml and the intra- and inter- day coefficient of variations (CV) and accuracy were ≤5.0% and ≤10.1%, ≥90% and ≥92%, respectively. Mean extraction recovery of omeprazole and the IS from plasma samples both was 91% and 91%, respectively. In processed samples, omeprazole was stable for at least 24 hours at room temperature (≥91%) and 48 hours at -20 º C (≥89%). In unprocessed samples it was stable at least 12 weeks at -20ºC (≥96%) and after 3 cycles of freeze and thaw (≥96%). Further, it was successfully applied to measure omeprazole level in samples obtained from a healthy volunteer.

Keywords: Omeprazole, Lansoprazole, Human plasma, HPLC.

[Full Text Article]



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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database