HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE PLANT OF PHYLLANTHUS NIRURI AGAINST CCl4 INDUCED LIVER DAMAGE IN WISTAR RATS

Daimu Basumatary and M. T. Sulthana*

ABSTRACT

The present study received an ethanolic extract of Phyllanthus Niruri (EEPN) to Wistar rats induced with carbon tetrachloride (CCl4) intraperitoneally (i.p.). This study examined the hepatoprotective properties of the whole plant extract to its ability to protect the liver. The administration of Silymarin at 40 mg/kg of b.w. is a standard treatment. The Wistar rats received EEPN, ranging from 200 mg/kg to 400 mg/kg over 20 days. They tested haematological factors such as haemoglobin (HG), red blood cells (RBC), and white blood cells (WBC), as well as biochemical factors such as serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin (TB), total cholesterol (TC), and triglycerides (TG). Ex vivo tests are used to determine the liver's weight and volume. The hepatic parameters of oxidative stress, namely glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT), were evaluated. After the experiment, the animals were euthanized, and their livers were extracted and washed with saline solution. The retrieved liver tissue was preserved in formalin at a concentration of 10% for histopathological analysis. The statistical analysis of the data was performed using a one-way analysis of variance (ANOVA), followed by a Tukey post hoc multiple comparison test.

Keywords: Phyllanthus Niruri, Hepatoprotective activity, CCl4, Silymarin, and Oxidative stress studies.