AMYLASE GLUCOSIDASE INHIBITORY AND ANTIOXIDANT ACTIVITIES OF PHASEOLUS VULGARIS SEED EXTRACTS BY INVITRO
B. Saritha*, S. K. Godasu, D. Varun, K. Archana, S. Priyanka, Bhavani Shankar and Anil
ABSTRACT
The main objective of the present study is to evaluate the hypoglycaemic activity of Phaseolus Vulgaris. seeds using various in vitro models like α-amylase and α-glucosidase inhibitory effects and antioxidant activity. Phytochemical screening of fruit and seed extracts revealed the presence of flavonoids and phenolics, accounting for its antidiabetic and antioxidant properties. Flavonoids are a group of polyphenolic compounds which exhibit several biological effects such as anti- inflammatory, antihepatotoxic, antiulcer activities, antidiabetic, antithrombotic etc. They also inhibit enzymes such as aldose reductase and xanthine oxidase. The in vitro antidiabetic activity of PVSP has been evaluated by measuring its α amylase and α glucosidase inhibitory activities and was compared with the standard drug acarbose, which is specific inhibitor of α glucosidase. Acarbose also possess inhibitory action of α amylase. The amount of glucose produced by the action of α glucosidase is estimated by using the enzyme glucosidase and peroxidase in vitro. However, the α glucosidase inhibition of the extract was much lesser than the inhibitory action shown by acarbose.
Keywords: Glucosidase Inhibitory, Antioxidant Activities, Phaseolus Vulgaris, Seed Extracts.
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