PHYTOCHEMICAL SCREENING AND INVITRO ANTI CANCER ACTIVITY OF ETHANOL AND AQUEOUS EXTRACTS OF COLDENIA PROCUMBENS LINN.

Nimma Vijayarekha*, S. K. Godasu, D. Varun, O. Vanaja, T. Jhansi, R. Raju, B. Shyamala and V. Sravan Kumar

ABSTRACT

The main aim of present work evaluation of invitro anti-cancer activity of ethanol and aqueous extracts of Coldenia Procumbens linn. Extracts were subjected to various qualitative phytochemical tests to identify the active constituents which showed presence of Alkaloids, Glycosides, Saponins, Carbohydrates, Tannin & Phenolic compounds, Triterpenoids, Steroids, Flavonoids, Fixed oil and fats. Ethanolic extract showed the presence of Alkaloids, Glycosides, Saponins, Carbohydrates, Tannins & Phenolic compounds Triterpenoids, Steroids, Flavonoids, Fixed oil and fats, Gum and mucilage and Lignin’s, whereas the aqueous extract showed, presence of Glycosides, carbohydrates, Tannin, Phenolic compounds, Flavonoids and Lignin’s. The ethanolic and aqueous extracts of Coldenia procumbens found to possess moderate cytotoxic potential with reference to the standard drug Methotrexate against MCF-7 breast cancer cell line. Among the extracts, the ethanolic extract showed better activity than the aqueous extract when comparing with standard. The reported cytotoxic activity of the plant extracts in the present study may be due to the presence of phenolic and flavonoid constituents. This indicates the possibility of the plant extracts investigated for further development to cancer therapeutic agent and warrants further studies to understand the mechanisms of cytotoxic activity of the plant extract Coldenia procumbens.

Keywords: Evaluation, Invitro, Anti-Cancer Activity, Ethanol And Aqueous Extracts, Coldenia Procumbens Linn.