DIGITAL RADIOGRAPHY AND VITAL STAINING AS DIAGNOSTIC TOOLS TO ASSESS BONE REGENERATION: IN VIVO STUDY
Mohamed S. Ayoub, Effat A. Abbas, Mohamed A. Abd El Hamid4, Houry M. Baghdadi, Dina S. Abd El Fattah and Marwa M. Ellithy*
Background /aim: The field of tissue engineering has developed over the past decade to recreate functional, healthy tissues and organs in order to replace diseased, dying or dead tissues. Digital radiography is a reliable and versatile technology that expands the diagnostic and image referral possibilities of radiology. The objective of this study is to evaluate the efficacy of DIGORA and double tetracycline labeling test as diagnostic aids in bone regeneration assessment. Methods and Material: Seven dogs were used for the study. Three critical sized calvarial defects were created in each dog. One defect was filled with autologous stem cells seeded on chitosan scaffold and soaked in osteogenic media, the second was filled with stem cells seeded on chitosan scaffold and soaked with Alendronate 10mg/ml and the third one is filled with stem cells seeded on chitosan scaffold. The rate of bone formation was tested using Digora and tetracycline double labelling test after eight weeks in each defect. Results: Both techniques showed enhanced bone formation in groups (A) & (B) compared to group (C). This was consistent with the H & E results published by our group in a previous study. Conclusions: DIGORA as a digital radiography tool and vital staining with double injection of tetracycline are reliable diagnostic and prognostic methods for assessing new bone formation.
Keywords: Digora, digital radiography, double tetracycline labeling test, bone regeneration, stem cells, chitosan, scaffold.
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