ANALYSIS OF MOLECULAR GENETIC STUDIES IN CHILDREN WITH CHRONIC BRONCHITIS

Sultanov Z. F., Musakhodjaeva D. A.*, Shamsiev F. M., Fayzullaeva N. Ya. and Masaidova I. B.

ABSTRACT

Background: In order to determine the role of polymorphic variants of the tumor necrosis factor α gene (TNFα -308G→A) in the development and course of chronic respiratory diseases, and analysis the frequency and alleles in patients with bronchial asthma and chronic bronchitis and healthy individuals of the Uzbek population was carried out. Objective: 116 children aged 4 to 14 were examined. According to the results of the survey, groups of patients with chronic bronchitis (CB) (n=42), BA (n=28) and a control group (n=46) were formed. Methods: For typing the candidate gene TNFa (-308G>A), pyrosequencing methods (PyroMark Q24, PyroMark Gold Q24 Reagents, Qiagen, Germany), qPCR method (DT-Prime, Russia) and microarray PCR detection method (MCE 202 MultiNA, Zhimadzu, Japan). Results: Analysis of the frequency distribution of genotypes and alleles of the studied gene showed that A allele and the A/G genotype of TNFα rs1800629 gene promoter predispose to the development of chronic bronchopulmonary pathology.

Keywords: Chronic bronchitis, Bronchial asthma, TNF ? -308 G/A gene polymorphism.