A STUDY ON THE EFFECT OF ARSENIC ON TISSUE HISTOLOGY AND ITS DEPOSITION PATTERN IN VARIOUS ORGANS OF WISTAR ALBINO RAT

Dil Umme Salma Chowdhury, Saiful Islam, Rasheda Akter, Laila Khaleda, Zillur Rahman, Mohammad Al-Forkan*

ABSTRACT

A clear and better knowledge on the effect of arsenic in hepatic, renal, splenic and cardiac tissue is required to understand the mechanism of arsenic toxicity. Our study was carried out to evaluate the arsenic-induced histological alterations observed in liver, kidney, spleen and heart of Wistar albino rats along with some hematological, biochemical and microscopic parameters. Wistar albino female rats were divided into four groups and treated as follows: Group 1: control, 2: arsenic (sodium arsenite: 50 ppm; 28 days), 3: arsenic (sodium arsenite: 100 ppm; 28 days), 4: arsenic (sodium arsenite: 150 ppm; 28 days). We found immunosuppression, leukopenia as well as marked increase in serum ALT and AST of arsenic intoxicated rats. Moreover, sodium arsenite produced marked necrosis in kidney of rats, whereas in heart mild muscle necrosis was observed. In case of spleen, moderately enlarged white pulp was seen. Besides, we also have found that liver and spleen accumulated more arsenic than kidney and heart. We believe that in order to know more extensively the toxic effects of arsenic, the findings of our study in animal model will become useful.

Keywords: Arsenic, histopathology, hematology, Wistar rat.