EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

MICROPROPAGATION, PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF A WILD EPIPHYTIC ORCHID ACAMPE PRAEMORSA (ROXB) OF KANYAKUMARI DISTRICT, INDIA

R. Mary Suja* and B. Christudhas Williams

ABSTRACT

Orchids are most abundant in the forest of Western Ghats of India have been threatened in their natural habitat due to habit dilapidation and anthropogenic activities. The scarcity of pollinators and poor seed setting are the major constraints in the natural propagation, leading to a continuous depletion of its natural population. Propagation from seeds is held back by low germination and survival rates due to the inept environmental conditions as a result their wild populations are diminishing at an alarming rate. The large-scale production of Acampe praemorsa (Roxb) requires efficient invitro propagation techniques to avoid overexploitation of natural populations. The immature embryos were inoculated on MS media, along with and devoid of different growth additives. The culture seeds showed positive germination response in the nutrient media but the frequency and onset of germination response and associated morphogenetic changes leading to seedling development varied with the nature of growth stimulus. In the basal MS medium 43.75±0.75% seeds were germinated (control). MS media supplemented with coconut water (CW) (15%), supported highest germination (70.75±0.75%) induced protocorm multiplication and complete seedlings were obtained in 131.50±1.73 days. Additional presence of activated charcoal (AC) (0.2%) in the MS medium inhibited the seed germination, while use of coconut water (15%) or (2 g/L) in the medium, favoured enhance early germination response and differentiation of protocorms.

Keywords: Acampe praemorsa, phytochemicals, antioxidant, micropropagation.


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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia