INVESTIGATION OF ANTI-SPASMODIC POTENTIAL OF CLEOME GYNANDRA ON ISOLATED CHICKEN INTESTINE

Sathya B.*, Rajesh M., Renuka M. and Rukshana Begum N.

ABSTRACT

The present study was aimed to investigate the anti-spasmodic effect of Cleome gynandra leaves of the family Cleomaceae on isolated chicken ileum by an in vitro pharmacological assay. Cleome gynandra is richly obtainable throughout India with countless hidden therapeutic values. It is used in traditional system for epilepsy, protozoal and worm infections, irritable bowel syndrome and as an antioxidant. The spasm was induced by acetycholine, the spasmogen. The ethanolic extract of Cleome gynandra leaves was prepared by simple maceration. Anti-spasmodic activity was assessed by antagonistic method of bioassay on chicken ileum using Tyrode as physiological salt solution. Effects of acetylcholine and acetylcholine followed by ethanolic leaves extract were studied on isolated chicken ileum and compared with atropine, the standard anti-spasmodic agent. The extract revealed marked decrease in acetylcholine induced spasm (the cholinolytic like effect) on chicken ileum induced by acetylcholine. Action of flavanoids and steroids on M3 receptors in the chicken ileum granted to the effect. In conclusion, the ethanolic extract of leaves of Cleome gynandra plant manifested optimistic antispasmodic action on chicken ileum.

Keywords: Acetylcholine, Atropine, Anti-spasmodic, Cleome gynandra, Chicken ileum.