PHAMACOGNOSTICAL ANALYSIS AND INVIVO ANTILITHIATIC ACTIVITY OF HYDRO ALCOHOLIC EXTRACT OF CANAVALIA GLADIATE AGAINST ETHYLENE GLYCOL INDUCED LITHIASIS IN WISTAR RATS
G. Nalini*, N. Chidambaranathan, T. Manibharathi, K. Marikrishnaa and G. N. Rishi.
The objective of the study is to investigate pharmacognostical analysis & antiurolithiatic activity of Hydroalcoholic extract of Cannavalia Gladiata. Pharmacognostic study include determination of physiochemical, fluorescence analysis & phytochemical screening of cannavalia gladiate. For antilithiatic study, 30 male wistar rats were segregated into 5 groups. All groups receive 1% Ethylene glycol for 28 days except normal control. G1(Normal control) receive normal diet and water, G2 (Toxic control) receive only ethylene glycol, G3(Standard) treated with Cystone at 100mg/kg whereas G4 & G5 treated with 200 & 400mg/kg of Hydroalcoholic extract of Cannavalia gladiata. After 28 days, various parameter like calcium, phosphate, magnesium, oxalate, protein, uric acid and creatinine concentration were estimated using Serum & Urine analysis by standard methods. Histopathological studies were carried out using Kidney section. Treatment with Hydroalcoholic extract of Canavalia gladiate at 200 and 400 mg/kg significantly reduced the serum & urinary levels of protein, uric acid, calcium, phosphate, oxalate & creatinine in dose dependent manner along with significant increase in magnesium. The histopathological examinations revealed that deposition of calcium oxalate crystals in renal tubules and glomerular congestion were reduced after HAECG treatment. This study demonstrate that Hydroalcoholic extract of Cannavalia gladiate reduces and inhibits the growth of urinary stones showed potent antiurolithiatic activity.
[Full Text Article]