FLAVONE C-GLYCOSIDE AND A NEW TANNIN ISOLATED FROM THE LEAVES OF NEOCARYA MACROPHYLLA (SABINE) PRANCE (CHRYSOBALANACEAE) USING CENTRIFUGAL PARTITION CHROMATOGRAPHY

Diara Diatta, Oumar Sambou, Philomène Akoua Yao-Kouassi, Isabelle Lachaise, Michael Rivard, Charlot Diatta, Firmin Sylva Barboza, Guata Yoro S. Y. and Abdoulaye Gassama*

ABSTRACT

Neocarya macrophylla (sabine) Prance is a plant of the traditional senegalese pharmacopoeia. Its leaves are used in the treatment of various diseases. The phytochemical investigation of its leaves resulted in the isolation of the new tannin (1), 1-O-galloyl-6-O-lutéoyl R-glucose (2) and Isoorientine (3). Their structures were isolated and characterized by using a combination of centrifugal partition chromatography (CPC), NMR (1D and 2D) spectroscopic and mass spectrometry (ESI-MS) analyses, as well as by its comparison with literature values. This study constitutes the first phytochemical examination of the leaves of N. macrophylla.

Keywords: Neocarya macrophylla, Leaves, Centrifugal Partition Chromatography, Preparative Chromatography, Tannin, flavone C-glycoside, NMR (1D and 2D).