( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , 

 ISSN 2394-3211

Impact Factor: 6.222

 ICV - 79.57



Unmesh Keshwar*, Deepmala Matey, Soumit Pimplapure, Sharad Dhurde and B. K. Shrikhande


Background: Standardization of multicomponent herbal formulation with respect to its active marker compound is still a major challenge. Siddhayu Ayurvedic Research Foundation Pvt. Ltd. has standardized multicomponent herbal capsule formulation with respect to its active marker compounds. Objective: Objective of the present study was to standardize multicomponent herbal capsule formulation by evaluating identification of its active marker compounds simultaneously, purity and impurity. Methods: The standardization parameters such as organoleptic evaluation, Simultaneous identification of active marker compounds by HPTLC, Average weight, Uniformity of weight, Disintegration, Assay (Protein content), Heavy Metals, Microbiological tests for the multicomponent herbal capsule formulation were done. Simultaneous chromatographic separation was achieved by using Toluene: Ethyl Acetate: Methanol: Glacial acetic acid, 5.0: 2.5: 0.5: 0.5 (v/v/v/v) as mobile phase followed by comparing the Rf values and overlaying the spectra with standards. The method was validated according to International Conference on Harmonization guidelines. Results: The presence of active marker compounds Gallic acid, Withaferin A and Piperine in multicomponent herbal capsule formulation was confirmed simultaneously by comparing the Rf values and overlaying the spectra with standards. Identification of Spirulina was confirmed in capsule by the fingerprint profile comparisons with that of reference Spirulina. All the test results of the formulated capsule evaluated were found well within the limits. Conclusion: The developed identification method by HPTLC was found to be simple, rapid, cost effective and able to identify the active marker compounds simultaneously, hence can be routinely employed for analysis in Quality Control Lab.

Keywords: Gallic acid, Withaferin A, Piperine, Spirulina, multicomponent immunity booster capsule, HPTLC.

[Full Text Article]



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Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database