EXTRACTION, ISOLATION AND ANTI-MICROBIAL SCREENING OF FLAVONOID CONSTITUENT FROM THE LEAVES OF MORINDA CITRIFOLIA L.

Chakravarthi K. S. S. and Suresh Kumar K.*

ABSTRACT

Natural products have numerous secondary metabolites that have their own pharmacological and biological activity. Different classes of phytoconstituents such as alkaloids, flavonoids, carbohydrates, tannins, steroids, terpenoids etc. are present. Flavonoids are the large group of phytonutrients of polyphenol class and generally flavonoids have 15 carbon skeleton structure. Isolation of a specific constituent from a plant extracts are still a tedious process which can be made simple by chromatographic process such as TLC. Morinda citrifolia L. was native to pacific societies and it comes under Rubiaceae family. It is commonly known as Noni. Noni is widely used for many pharmacological uses such as immunomodulatory, anti-cancer, anti-diabetic, gastric ulcer healing etc. In the present study an attempt has been made to identify the flavonoid constituent that is being present in the leaf of the plant by HPTLC. Further, the isolation of the same was performed using preparative TLC using solvent system of Chloroform: Ethyl acetate in the ratio 50:50. Confirmation of the isolate was done by HPTLC. The anti-microbial screening of the isolate was also performed by Disk diffusion and broth dilution method. The flavonoid constituent was isolated and anti-microbial screening was performed in 10 different test organisms. This strategy could be used to isolate constituents from the plant extracts by this we can get numerous informations.

Keywords: Preparative TLC; Morinda citrifolia; Isolation; Anti-microbial screening.