EVALUATION OF THE ANTIMICROBIAL EFFICACY OF OZONATED WATER AND THE EFFECT ON COLOR STABILITY OF DENTURE BASE RESIN: AN IN VITRO STUDY
*Dr. Amogh Gulwe, Dr. Samarth Kumar Agarwal, Dr. Rachna Maheshwari, Dr. Romil Singhal
Purpose: The purpose of this study was to evaluate antimicrobial efficacy of ozonated water on heat cure polymethyle methacrylate denture base acrylic resin and its color stability before and after use of ozonated water. Materials and method: Ninety Heat cure Polymethyle methacrylate denture base acrylic resin samples having 25x10x3 mm of dimension were fabricated. These samples were infected with the Candida albicans and treated with ozonated water with three concentrations (2mg/L, 4mg/L, 6mg/L) at three time intervals (5, 10, 30 minutes). After that Colony of candida albicans counted and color stability checked with spectrophotometer. Results: One-way ANOVA showed highly statistically significant difference in 2mg/L, 4mg/L and 6mg/l concentration of ozonated water on Candida albicans at 5, 10, and 30 minutes (p<0.001). Reduction of count of Candida albicans seen in all groups but 6mg/L at 30 minutes of time interval showed marked reduction of count of Candida albicans. While analysing color stability result showed that Tukey’s post hoc test was statistically significant in all groups (p=0.004). Conclusion: Ozonated water was very effective for reducing Candida albicans count on heat cure polymethyle methacrylate denture base acrylic resin samples. Significant color change was seen on heat cure denture base acrylic resin samples after use of ozonated water.
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