EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

European Journal of Pharmaceutical and Medical Research (EJPMR) has indexed with various reputed international bodies like : Google Scholar , Indian Science Publications , InfoBase Index (In Process) , SOCOLAR, China , Research Bible, Fuchu, Tokyo. JAPAN , International Society for Research activity (ISRA) , Scientific Indexing Services (SIS) , Polish Scholarly Bibliography , Global Impact Factor (GIF) (Under Process) , Universal Impact Factor , International Scientific Indexing (ISI), UAE  , Index Copernicus , CAS (A Division of American Chemical Society) USA (Under Process) , Directory of Open Access Journal (DOAJ, Sweden, in process) , UDLedge Science Citation Index , CiteFactor , Directory Of Research Journal Indexing (DRJI) , Indian citation Index (ICI) , Journal Index (JI, Under Process) , Directory of abstract indexing for Journals (DAIJ) , Open Access Journals (Under Process) , Impact Factor Services For International Journals (IFSIJ) , Cosmos Impact Factor , Jour Informatics (Under Process) , Eurasian Scientific Journal Index (ESJI) , International Innovative Journal Impact Factor (IIJIF) , Science Library Index, Dubai, United Arab Emirates , Pubmed Database [NLM ID: 101669306] (Under Process) , IP Indexing (IP Value 2.40) , Web of Science Group (Under Process) , Directory of Research Journals Indexing , Scholar Article Journal Index (SAJI) , International Scientific Indexing ( ISI ) , Scope Database , Academia , 

 ISSN 2394-3211

Impact Factor: 7.065

 ICV - 79.57

Abstract

DISTRIBUTION OF AMPC, SHV AND CTX-M GENES IN MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA FROM CLINICAL SPECIMENT IN UNIVERSITY OF PORT HARCOURT TEACHING HOSPITAL RIVERS STATE, NIGERIA

Kingsley Excel Dunga*, Karibo George Furo, Abimbola Temitayo Awopeju, Chinyere Ihuarulam Okoro, Y. M. Tatfeng, Nnamdi Jude Ofoegbu, J. O. Njoku, Ezinne Jessica Ekeleme and C. N. Ohalete

ABSTRACT

Antibiotic resistance is a global public health concern and occurs through several defined mechanisms. However, Pseudomonas species have been said to have genes that code for beta-lactamase production, some of such genes are AMPC, SHV and CTX-M. This study was aimed at detecting AMPC, SHV and CTX-M genes in multi- drug resistant Pseudomonas aeruginosa. The isolates were cultured and phenotypically identified by Gram staining and biochemical testing. DMA extraction was carried Qutiby boiling method and gene detection was done. Two hundred and thirty five (235) clinical samples collected from University of Port Harcourt Teaching Hospital (UPTH) were cultured on blood agar and MacConkey agar and incubated at 37°C for 24hours. 135 organisms were isolated consisting 81(60%) of Pseudomonas aeniginosa. The distribution of the isolates by specimen revealed that, 32(23.7%), 2(1.5%), 37(27.4%), 6(4.4%), 2(1.5%) and 2(1.5%) were from urine, ear, wound, blood culture, abdominal fluid and pleural fluid respectively. Out of the six antibiotics used in this study, Ceftriaxone and Cefuroxime were the most resistant 75(95.6%). The distribution of resistance genes showed that Molecular characterization with 16s rRNA markers revealed that some isolates that were phenotypically characterized as Pseudomonas sp whereas it was Alcaligenes faecalis. The result showed the presence of Amp C and the absence of SHV and CTX-M gene among Pseudomonas isolates from UPTH, thus patients can be treated effectively by the knowledge of the drug mechanism.

Keywords: .


[Full Text Article] [Download Certificate]

Citation

  All Since 2019
 Citation  3086  2560
 h-index  18  17
 i10-index  64  44

INDEXING

NEWS & UPDATION

  • New Issue Published

    Its Our pleasure to inform you that, EJPMR 1 March 2024 Issue has been Published, Kindly check it on https://www.ejpmr.com/issue

  • EJPMR: MARCH ISSUE PUBLISHED

    MARCH 2024 issue has been successfully launched on MARCH 2024.

  • EJPMR New Impact Factor

    EJPMR Impact Factor has been Increased to 7.065 for Year 2024.

  • Index Copernicus Value

    EJPMR Received Index Copernicus Value 79.57, due to High Quality Publication in EJPMR at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Article are invited for publication in EJPMR Coming Issue

Google Scholar Indian Science Publications InfoBase Index (In Process) SOCOLAR, China Research Bible, Fuchu, Tokyo. JAPAN International Society for Research activity (ISRA) Scientific Indexing Services (SIS) Polish Scholarly Bibliography Global Impact Factor (GIF) (Under Process) Universal Impact Factor International Scientific Indexing (ISI), UAE Index Copernicus CAS (A Division of American Chemical Society) USA (Under Process) Directory of Open Access Journal (DOAJ, Sweden, in process) UDLedge Science Citation Index CiteFactor Directory Of Research Journal Indexing (DRJI) Indian citation Index (ICI) Journal Index (JI, Under Process) Directory of abstract indexing for Journals (DAIJ) Open Access Journals (Under Process) Impact Factor Services For International Journals (IFSIJ) Cosmos Impact Factor Jour Informatics (Under Process) Eurasian Scientific Journal Index (ESJI) International Innovative Journal Impact Factor (IIJIF) Science Library Index, Dubai, United Arab Emirates Pubmed Database [NLM ID: 101669306] (Under Process) IP Indexing (IP Value 2.40) Web of Science Group (Under Process) Directory of Research Journals Indexing Scholar Article Journal Index (SAJI) International Scientific Indexing ( ISI ) Scope Database Academia