DISTRIBUTION OF AMPC, SHV AND CTX-M GENES IN MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA FROM CLINICAL SPECIMEN IN UNIVERSITY OF PORT HARCOURT TEACHING HOSPITAL RIVERS STATE, NIGERIA
Kingsley Excel Dunga, Karibo George Furo, Abimbola Temitayo Awopeju, Chinyere Ihuarulam Okoro, Y.M. Tatfeng, Nnamdi Jude Ofoegbu, J.O. Njoku, Ezinne Jessica Ekeleme, C.N Ohalete
Antibiotic resistance is a global public health concern and occurs through several defined mechanisms. However, Pseudomonas species have been said to have genes that code for beta-lactamase production, some of such genes are AMPC, SHV and CTX-M. This study was aimed at detecting AMPC, SHV and CTX-M genes in multi-drug resistant Pseudomonas aeruginosa. The isolates were cultured and phenotypically identified by Gram staining and biochemical testing. DMA extraction was carried Qutiby boiling method and gene detection was done. Two hundred and thirty five (235) clinical samples collected from University of Port Harcourt Teaching Hospital (UPTH) were cultured on blood agar and MacConkey agar and incubated at 37°C for 24hours. 135 organisms were isolated consisting 81(60%) of Pseudomonas aeniginosa. The distribution of the isolates by specimen revealed that, 32(23.7%), 2(1.5%), 37(27.4%), 6(4.4%), 2(1.5%) and 2(1.5%) were from urine, ear, wound, blood culture, abdominal fluid and pleural fluid respectively. Out of the six antibiotics used in this study, Ceftriaxone and Cefuroxime were the most resistant 75(95.6%). The distribution of resistance genes showed that Molecular characterization with 16s rRNA markers revealed that some isolates that were phenotypically characterized as Pseudomonas sp whereas it was Alcaligenes faecalis. The result showed the presence of Amp C and the absence of SHV and CTX-M gene among Pseudomonas isolates from UPTH, thus patients can be treated effectively by the knowledge of the drug mechanism.
Keywords: Genes, Beta lactam resistant, klebsiella, New Delhi metallo.
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