IN VITRO ANTACID EFFECTS OF KHAYA GRANDIFOLIOLA (MELIACEAE) AND CURATIVE EFFECTS FORMULATED WITH CLAY (MY41G) ON CHRONIC GASTRIC ULCERS IN RATS

Essama M. D. Sandrine*, Ndji Otto Gustave Lebeau, Emakoua Joseph Fleurie and Tan Paul Vernyuy

ABSTRACT

Aim: The current study investigated the in vitro antiacide and in vivo curative actions of aqueous extract of Khaya grandifoliola + MY41gc clay in rats. Materials and Methods: This study evaluated possible antacid action with the neutralizing effect (in vitro) of the extract against pylorus ligation; and curative effects of Khaya grandifoliola combined with MY41gc clay on chronic gastric ulcers in rats. Chronic gastric ulcers were induced by injecting 0.05 mL of acetic acid (30 %) into the stomach wall. From day 5-10 after induction of ulcers, rats were treated daily with the mixture of K. grandifoliola + MY41gc clay (250 + 250 and 250 + 500) mg/kg. The ulcer index, percentage of healing, mucus secretion, histological parameters, and oxidative stress parameters were assessed. In vitro, K. grandifoliola had antacid and neutralizing effects practically similar of sodium bicarbonate. Curative treatment with K. grandifoliola + MY41gc clay solution for 10 days resulted in accelerated spontaneous healing of chronic gastric ulcers in both formulation (66.26-100 %) and promoted significantly higher levels of catalase and highly decreased levels of MDA in rats. Conclusion: K. grandifoliola possess neutralizing effects and his formulation with MY41gc clay has curative actions in rats.

Keywords: Neutralizing effect, chronic gastric ulcers, MY41gc clay.