CYTOTOXIC EFFECT OF ETHANOLIC EXTRACT OF CRATAEVA RELIGIOSA G. FORST LEAVES (CAPPARACEAE)
Mohanapriya R.*, T. Venkata Ratina Kumar, Maruthamuthu Murugesan and Sesha Kumar S. S.
The Capparaceae, often known as Capparidacea or the Caper family, is the group of plants that includes Crataeva religiosa. The plant is known by the scientific name Crataeva, which honours the Greek botanist Crataevus who lived during the time of Hippocrates. The botanical name religiosa denotes the plant's profusion close to places of devotion. Examining Crataeva religiosa 's cytotoxic potential against HepG2 cell lines was the goal of the current investigation. The Crataeva religiosa leaves were gathered, and an ethanolic extract of the leaves was made, which was used to assess the cytotoxicity. Doxorubicin and 10% dimethyl sulfoxide were used as positive and negative controls, respectively, in the MTT experiment to assess the cytotoxic activity. HepG2 cell viability was considerably decreased by ethanol extracts of Crataeva religiosa leaves at concentrations of 10, 20, 40, 80, 160, and 320 g/ml. The IC50 value for doxorubicin and the ethanolic extracts of the leaves was computed using Graph Pad Prism, and it was discovered to be 18.52 g/ml and 51.61 g/ml, respectively. Due to the presence of quercetin in the extract, this study found that EECRL demonstrated considerable in vitro anticancer activity when compared to doxorubicin against HepG2 cell lines. Due to the fact that quercetin can cause HepG2 cells to apoptosis by reducing the expression and inhibiting intracellular FASN activity.
Keywords: MTT assay, Cytotoxicity, HepG2, Crataeva religiosa, Quercetin.
[Full Text Article]