ISOLATION AND IDENTIFICATION OF SOME ENTEROBACTERIAL SPECIES FROM CHILDREN WITH DIARRHOEA WHOM ATTENDING THE AL-GOURDA HEALTH CENTRE IN SABHA CITY AND STUDYING THEIR ABILITY TO PRODUCE PROTEASE ENZYME
Mohamed F. M. Elbreki*, Hadera F. M. Albreki, Suliman. A.A. Elgadi and Rawan M. F. Elbreki
ABSTRACT
This study included the isolation and identification of some bacterial species causing diarrhea in children under five years old. Ninety eight stool samples were collected from children attending the Al-Gourda Health Center in Sabha for the period between February 2022 to September 2022. Samples were cultured on selected media and the growing colonies were identified using morphological characteristics and biochemical tests. fifty eight bacterial isolates belongs to the family enterobacteriaceae were identified, 46.6 %( 27 isolates) of them were E.coli; 20.7% (12 isolates) were Salmonella; 15.5 %( 9 isolates) of Shigella; 10.3% (6 isolates) of Klebsiella; 5.1% (3 isolates) and 1.7% (one isolates) of Pseudomonas. Protease production, which is considered as one of the factors enhancing inflammatory bowel disease, was investigated and it has been found that all the isolates used in the study were able to produce the enzyme.
Keywords: Bacteria, Children, Diarrhea, Al-Gourda Health Center.
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