FORMULATION AND CHARACTERIZATION OF TOPICAL LYCOPENE PHYTOSOMES FOR IMPROVED PERMEATION

Viresh K. Chandur* and Ramkrishna Shabaraya A.

ABSTRACT

Objective: An attempt was made to develop topical delivery of lycopene for its beneficial use as an anti-oxidant by phytosome technology. Material & Method: Phytosomes were prepared by refluxing or dissolving different ratios of lycopene in chloroform or hexane and Phospholipid in dichloromethane involved precipitation sonication method. 1% equivalent complex was placed in three different formulations of Cream and hydrophilic gel bases made out of steric acid and Carbopol respectively for better utility. Formulations were evaluated for appearance, pH, viscosity, Spreadability, Extrudability, washable property and in vitro diffusion to check for its stability. Results & Discussion: 1: 1 molar ratio was found to be the best phytosomal complex with entrapment efficiency of 83.22±0.54 % and physicochemical parameters showed acceptable results for both creams and gels for pH in range of 6.4±0.043 to 6.95±0.041 compatible to skin pH with less irritation. Viscosity 3.28±44.04 kP for cream and Gel 5.8±56.88 kP, Spreadability 14.66±0.04 g*cm/sec for cream and gel 36.22±0.04 g*cm/sec and % decrease Extrudability for cream 99.17±0.05 and gel 99.84±0.04 respectively showed inter related parametric performance for better results. In- vitro studies were found to be fare release up to 95% for creams and gels at the end of 5 h. Stability studies was carried out at two different temperature at 30 o C ±0.5oC and 65% RH±2 RH and 4 o C ±0.5oC and 75%RH for 90 days and found to be stable.

Keywords: Lycopene, Phytosomes, Spreadability, Extrudability, Skin irritation.