CHARACTERIZATION OF SOLANUM NIGRUM L. VARIANTS BACK AND ORANGE FRUITS BY RAPD MARKER
G. Jayanthi*
ABSTRACT
Randaom Amplified Polimorphic DNA (RAPD) technique was used as a tool for assessing genetic diversity and species relationship among two variants of Solanum nigrum (black and orange fruited). Four primers were used in this study. A total of 48 bands were observed among the variants of Solanum nigrum L. In which 8% were polymorphic among the S. nigrum variants whilst, 92% of these bands were shared by the two variants. A total of 25 and 23 DNA products were scored for Solanum nigrum black and orange fruited respectively. Genetically distinct genotypes identified using RAPD markers could be potential sources of germplasm for Solanum nigrum improvement. Thus, RAPD markers combined with morphological analysis proved to be a quick, simple and significant testing methods to assess genetic diversity among S. nigrum variants (black and orange fruited).
Keywords: Solanum nigrum, black nightshade, Solanaceae, Randaom Amplified Polimorphic DNA, Genetic diversity.
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