INHIBITORY ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME FROM GOAT MILK PROTEIN
Man Kyu Huh* and Yong Lim
Goat milk is the milk of domestic goats. Due to unavailability of cow milk, goat milk and its products are important daily food sources of protein. Angiotensin converting enzyme (ACE) inhibitors plays a critical role in treating hypertension. The ACE activity was evaluated by determining the hydrolysis rate of substrate, hippuryl-L-histidyl-L-leucine (HHL), using captopril. Four products were arbitrarily selected that are sold in Korea because they advertised and contained the most goat milk protein ingredients. Water, ethanol, and methanol were used as the extraction solvent. Hot water extract of the goat's milk protein was evaluated 42.7% on 50 mM and that of the ethanol extract was 40.1% at same concentration. Methanol extract was 39.3% at same concentration. ACE inhibitory activity was no significant difference among four products at low concentrations (25, 12.5, and 6.25 mg/L) (p > 0.05). However, there was significant difference among 50 mg/L (p < 0.05). We prepared four products as ACE inhibitor and compared its positive control inhibitory activity with captopril. Products A, B, C, and D were 42.7%, 40.1%, 39.3%, and 39.1% on the concentration of 50 mg/L, respectively. Overall, goat milk proteins of four products were not enough to generate the inhibitory activity of ACE. KEYWORDS: Angiotensin converting enzyme (ACE), Goat milk protein, extraction solvent.
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