INVESTIGATION OF THE EFFECT OF BIOACTIVE COMPOUND ISOLATED FROM SEEDS OF PSORALEA CORYLIFOLIA L. ON DNA OF PATHOGENIC BACTERIA
Kiran B.*, Lalitha V. and Raveesha K. A.
Four species of bacteria viz., Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Xanthomonas sp was treated with 250, 500, 1000 and 1500ppm concentration of the bioactive compound 2H-Furo[2,3-H]-1-benzopyran-2-one isolated from the seeds of Psoralea corylifolia for 24 hours and DNA bands was observed in all the treated bacteria by agarose gel electrophoresis. In control without any treatment of bioactive compound, E. coli, S. aureus, B. subtilis and Xanthomonas sp. recorded 12, 14, 14 and 13 DNA bands respectively. In treated, E. coli, recorded 14, 15, 16 and 16 at 250, 500, 1000 and 1500ppm concentration, S. aureus recorded 14, 15, 16 and 16, B. subtilis recorded 15, 16, 17, 17 and Xanthomonas sp. recorded 13, 14, 15 and 16 at 250, 500, 1000 and 1500ppm concentration of the bioactive compound respectively.
Keywords: Psoralea corylifolia, bioactive compound, Bacteria, DNA.
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